The UAP1 Monoclonal Antibody (CAB8662) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the UAP1 protein, it allows for accurate detection and analysis in a variety of cell types, enabling in-depth investigations into glycosylation pathways and related cellular processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UAP1 Monoclonal Antibody
SKU:
CAB8662
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1769
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AGX, AGX1, AGX2, SPAG2, UAP1
Positive Sample:
HepG2, DU 145, Jurkat, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
59kDa
Observed MW:
59kDa
Enables identical protein binding activity. Predicted to be involved in UDP-N-acetylglucosamine biosynthetic process. Located in cytosol; nucleoplasm; and plasma membrane.
Purification Method
Affinity purification
Gene ID
6675
RRID
AB_2863579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UAP1 Rabbit mAb (CAB8662) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using UAP1 Rabbit mAb (CAB8662) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
