Anti-Butyrly-HIST1H4A (K5) Antibody (PACO58647)

Antibody Name:	Butyrly-HIST1H4A (K5) Antibody (PACO58647)
Antibody SKU:	PACO58647
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IF, IP, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000, IF:1:1-1:10, IP:1:200-1:2000
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Butyrly-Lys (5) derived from Human Histone H4

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h. All lanes: HIST1H4A antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Predicted band size: 12 kDa. Observed band size: 12 kDa.
	Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). Lane 1: Rabbit control IgG instead of PACO58647 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO58647 (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg). Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg).
	Immunofluorescence staining of HepG2 cells (treated with 30mM sodium butyrate for 4h) with PACO58647 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms:

Histone H4, HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4, H4/A H4FA; H4/I H4FI; H4/G H4FG; H4/B H4FB; H4/J H4FJ; H4/C H4FC; H4/H H4FH; H4/M H4FM; H4/E H4FE; H4/D H4FD; H4/K H4FK; H4/N H4F2 H4FN HIST2H4; H4/O H4FO;

UniProt Protein Function:	Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:	Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular location: Nucleus. Chromosome. Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35 Sequence similarities: Belongs to the histone H4 family. Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]
UniProt Code:	P62805
NCBI GenInfo Identifier:	77539758
NCBI Gene ID:	554313
NCBI Accession:	NP_001029249.1
UniProt Secondary Accession:	P62805,P02304, P02305, Q6DRA9, Q6FGB8, Q6NWP7, A2VCL0
UniProt Related Accession:	P62805
Molecular Weight:	11,367 Da
NCBI Full Name:	histone H4
NCBI Synonym Full Names:	histone cluster 2, H4b
NCBI Official Symbol:	HIST2H4B
NCBI Official Synonym Symbols:	H4/o
NCBI Protein Information:	histone H4; histone 2, H4b
UniProt Protein Name:	Histone H4
Protein Family:	Histone
UniProt Gene Name:	HIST1H4A
UniProt Entry Name:	H4_HUMAN

Antibodies	ELISA Kits
Anti-Histone H4 Antibody (CAB1131)	Human Histone H4 (HIST1H4A) ELISA Kit
Anti-Acetyl-Histone H4-K12 Antibody (CAB14227)	Histone H4 Colorimetric Cell-Based ELISA Kit
Anti-Acetyl-Histone H4-K5 Antibody (CAB15233)
Anti-MonoMethyl-Histone H4-K5 Antibody (CAB17859)
Anti-MonoMethyl-Histone H4-K20 Antibody (CAB2370)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)