Description
CA5B Antibody (CAB17633)
The CA5B Antibody (CAB17633) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.CA5B plays a key role in catalyzing the reversible hydration of carbon dioxide to bicarbonate, which is essential for maintaining acid-base balance in cells. Its dysregulation has been implicated in various diseases, including metabolic disorders and cancer.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | CA5B Antibody |
| SKU: | CAB17633 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MVVM NSLR VILQ ASPG KLLW RKFQ IPRF MPAR PCSL YTCT YKTR NRAL HPLW ESVD LVPG GDRQ SPIN IRWR DSVY DPGL KPLT ISYD PATC LHVW NNGY SFLV EFED STDK SVIK GGPL EHNY RLKQ FHFH WGAI DAWG SEHT VDSK CFPA ELHL VHWN AVRF ENFE DAAL EENG LAVI GVFL KLGK HHKE LQKL VDTL PSIK HKDA LVEF GSFD PSCL MPTC PDYW TYSG SLTT PPLS ESVT WIIK KQPV EVDH DQLE QFRT LLFT SEGE KEKR MVDN FRPL QPLM NRTV RSSF RHDY VLNV QAKP KPAT SQAT P | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | CAVB, CA-VB, CA5B |
| Positive Sample: | Mouse kidney, Rat kidney, Rat lung |
| Cellular Localization: | Mitochondrion. |
| Calculated MW: | 36kDa |
| Observed MW: | 32kDa |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes carbonic anhydrase 5B. CA5B, and the related CA5A gene, has its expression localized in the mitochondria though CA5B has a wider tissue distribution than CA5A, which is restricted to the liver, kidneys, and skeletal muscle. A carbonic anhydrase pseudogene (CA5BP1) is adjacent to the CA5B gene and these two loci produce CA5BP1-CA5B readthrough transcripts.
| Purification Method | Affinity purification |
| Gene ID | 11238 |
| RRID | AB_2768668 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using CA5B Rabbit pAb (CAB17633) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using CA5B Rabbit pAb (CAB17633) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
