The Calpain 2 Monoclonal Antibody (CAB4066) is a high-quality antibody developed for reliable detection and analysis of target proteins. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2863180 Gene ID 824 Swiss Prot Synonym CANP2; mCANP; CANPL2; CANPml; Calpain 2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calpain 2 Monoclonal Antibody
SKU:
CAB4066
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0891
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CANP2, mCANP, CANPL2, CANPml, Calpain 2
Positive Sample:
HeLa, Mouse lung, Mouse brain, Mouse kidney, Rat lung, Rat brain, Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
80kDa
Observed MW:
80kDa
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2863180 Gene ID 824 Swiss Prot Synonym CANP2; mCANP; CANPL2; CANPml; Calpain 2
Purification Method:
Affinity purification
Gene ID:
824
RRID:
AB_2863180
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Calpain 2 Rabbit mAb (CAB4066) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
