The CTBP2 Antibody (CAB16826) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CTBP2 protein, this antibody allows for the detection and analysis of CTBP2 expression in various cell types, making it an essential reagent for studies in molecular biology and cancer research.CTBP2, also known as C-terminal binding protein 2, is a transcriptional corepressor that plays a crucial role in regulating gene expression and cellular proliferation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CTBP2 Antibody
SKU:
CAB16826
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CTBP2
Positive Sample:
HeLa, COS-7, BxPC-3, NIH/3T3, Mouse brain, Rat brain
Cellular Localization:
Cell Junction, Nucleus, Synapse.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1488
RRID
AB_2769073
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CTBP2 Rabbit pAb (CAB16826) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using CTBP2 Rabbit pAb (CAB16826) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
