The Cyclophilin 40 Monoclonal Antibody (CAB5097) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. RRID AB_2863442 Gene ID 5481 Swiss Prot Synonym CYPD; CYP-40; Cyclophilin 40
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cyclophilin 40 Monoclonal Antibody
SKU:
CAB5097
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1260
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:4000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYPD, CYP-40, Cyclophilin 40
Positive Sample:
NIH/3T3, Jurkat, C6, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
41kDa
Observed MW:
41kDa
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. RRID AB_2863442 Gene ID 5481 Swiss Prot Synonym CYPD; CYP-40; Cyclophilin 40
Purification Method:
Affinity purification
Gene ID:
5481
RRID:
AB_2863442
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclophilin 40 Rabbit mAb (CAB5097) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Cyclophilin 40 Rabbit mAb (CAB5097) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
