The DHEA (Dehydroepiandrosterone) ELISA Kit is designed for the quantitative detection of Dehydroepiandrosterone (DHEA) levels in various biological samples. DHEA is an important endogenous steroid hormone involved in a variety of functions, including the production of adrenal androgens and sex steroids, as well as in the neurosteroid pathway, ultimately impacting cognition, mood, and physical health. It plays a significant role in the body's endocrine and immune systems, underpinning various physiological processes and has been implicated in age-related diseases, mood disorders, and autoimmune diseases. Accurate measurement of DHEA is essential for understanding its role and impact on health and disease as well as for developing targeted therapeutic strategies. Assay Genie's DHEA ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's DHEA ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
DHEA (Dehydroepiandrosterone) ELISA Kit
SKU:
AEES00004
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
