The FA-VB9 (Folic Acid Vitamin B9) ELISA Kit is a versatile and reliable kit designed for the accurate measurement of folic acid levels in biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Folic acid, also known as Vitamin B9, is an essential nutrient that plays a crucial role in DNA synthesis and repair, cell division, and overall cellular function. Adequate folic acid levels are important for overall health and can impact a range of conditions, including neural tube defects in pregnancy, cardiovascular diseases, and certain cancers. By accurately measuring folic acid levels in biological samples, researchers can gain valuable insights into the role of folic acid in health and disease, as well as develop potential therapeutic interventions. The FA-VB9 ELISA Kit is a valuable tool for studying folic acid metabolism and its implications for human health.
Product Name:
FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit
SKU:
UNES00006
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
