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Key Features

GeniePlex Human Th1/Th2/Th17 14-plex (96 Tests) (HUAMPM016)

Datasheet
SKU HUAMPM016
Product Type Multiplex
Reactivity Human
ELISA Type Multiplex
Analytes IFN-Gamma
Analytes IL-1-Beta
Analytes IL-2
Analytes IL-4
Analytes IL-5
Analytes IL-6
Analytes IL-8
Analytes IL-10
Analytes IL-12p70
Analytes IL-17A
Analytes IL-17F
Analytes IL-22
Analytes TNF-Alpha
Analytes TNF-Beta
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Description

system_update_altDatasheet
Product Name:GeniePlex Human Th1/Th2/Th17 14-plex (96 Tests)
Product Code:HUAMPM016
Reactivity:Human
Analytes:IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, TNF-Alpha, TNF-Beta
Sensitivity (LODs):IFN-Gamma: < 3 pg/mL,  IL-1-Beta: < 5 pg/mL,  IL-2: < 3 pg/mL,  IL-4: < 1 pg/mL,  IL-5: < 2 pg/mL,  IL-6: < 5 pg/mL,  IL-8: < 1 pg/mL,  IL-10: < 2 pg/mL,  IL-12p70: < 3 pg/mL,  IL-17A: < 3 pg/mL, IL-17F: < 2 pg/mL,  IL-22: < 3 pg/mL,  TNF-Alpha: < 1 pg/mL,  TNF-Beta: < 5 pg/mL
Sample types:Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Assay Type: Multiplex
Detection Method:Flow Cytometry
Assay Time: 2 hours
1x Ab-conjugated beads (premixed):S4P2 - anti-human TNF-Beta, S4P3 - anti-human TNF-Alpha, S4P6 - anti-human IL-1-Beta, S4P7 - anti-human IL-2, S4P8 - anti-human IL-17F, S4P9 - anti-human IFN-Gamma, S4P10 - anti-human IL-8, S4P12 - anti-human IL-12p70, S5P2 - anti-human IL-22, S5P4 - anti-human IL-4, S5P5 - anti-human IL-5, S5P6 - anti-human IL-6, S5P9 - anti-human IL-17A, S5P10 - anti-human IL-10
2x Biotin-detection Ab (premixed):Biotin - anti-human TNF-Beta, Biotin - anti-human TNF-Alpha, Biotin - anti-human IL-1-Beta, Biotin - anti-human IL-2, Biotin - anti-human IL-17F, Biotin - anti -human IFN-Gamma, Biotin - anti-human IL-8, Biotin - anti-human IL-12p70, Biotin - anti-human IL-22, Biotin - anti-human IL-4, Biotin - anti-human IL-5, Biotin - anti-human IL-6, Biotin - anti-human IL-17A, Biotin - anti-human IL-10
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix- Human TH1/Th2/Th17 14-plex. Vial A and Vial B containing lyophilized recombinant human IFN-Gamma, IL-1 Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, TNF-Alpha, TNF Beta.
LLOQs:IFN-Gamma: <10 pg/mL, IL-1-Beta:  <10 pg/mL, IL-2: <10 pg/mL, IL-4: <3 pg/mL, IL-5: <5 pg/mL, IL-6: <10 pg/mL, IL-8: <1 pg/mL, IL-10: <5 pg/mL,  IL-12p70: <10 pg/mL, IL-17A: <10 pg/mL, IL-17F: <5 pg/mL, IL-22: <10 pg/mL, TNF-Alpha: <2 pg/mL, TNF-Beta: <10 pg/mL.
ULOQs:IFN-Gamma: >2,000 pg/mL, IL-1-Beta: >5,000 pg/mL,  IL-2: >2,000 pg/mL, IL-4: >1,000 pg/mL, IL-5: >2,000 pg/mL, IL-6: >5,000 pg/mL, IL-8: >1,000 pg/mL,  IL-10: >5,000 pg/mL, IL-12p70: >2,000 pg/mL,  IL-17A: >1,000 pg/mL, IL-17F: >2,000 pg/mL, IL-22: >2,000 pg/mL, TNF-Alpha: >1,000 pg/mL, TNF-Beta:  > 5,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: <10%
Inter-assay CV:Inter-assay CV: <20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume:15 µL/test

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

A filter plate washer is required for the following protocol. For more information on GeniePlex click here.

StepProtocol
1.Prepare the filter plate template. Mark the standard, sample and blank wells. Standards and samples should be run in duplicates or triplicates. If the whole plate will not be used, seal the