The Galactosidase alpha (GLA) Antibody (CAB1700) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the GLA protein, researchers can accurately detect and analyze its expression in a variety of cell types.GLA, also known as alpha-galactosidase A, is essential for the breakdown of a specific type of lipid molecule called globotriaosylceramide. Deficiencies in GLA enzyme activity have been linked to Fabry disease, a rare genetic disorder characterized by the buildup of these lipid molecules in various tissues throughout the body.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Galactosidase alpha (GLA) Antibody
SKU:
CAB1700
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GALA, Galactosidase alpha (GLA)
Positive Sample:
SW620, BT-474, HeLa
Cellular Localization:
Lysosome.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Purification Method
Affinity purification
Gene ID
2717
RRID
AB_2763752
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Galactosidase alpha (GLA) Rabbit pAb (CAB1700) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Galactosidase alpha (GLA) Rabbit pAb (CAB1700). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
