The HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in WB, IHC-P, ELISA, DB applications and has demonstrated reactivity against Rabbit samples.
Product Name:
HRP-conjugated Goat anti-Rabbit IgG (H+L)
SKU:
CABS014
Size:
100μL
Reactivity:
Rabbit
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISADB
Recommended Dilution:
WB
1:2000 - 1:10000
DB
1:2000 - 1:10000
IHC-P
1:50 - 1:200
ELISA
1:5000 - 1:10000
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769854
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000. Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (CABS014) at 1:4000-1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
