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Human and Mouse Phospho-TYK2 (Y1054) PharmaGenie ELISA Kit (SBRS2009)

SKU:
SBRS2009
Product type:
ELISA Kit
Reactivity:
Human
Application:
ELISA
ELISA Type:
Sandwich
  • Human and Mouse Phospho-TYK2 Y1054 PharmaGenie ELISA Kit SBRS2009
  • Human and Mouse Phospho-TYK2 Y1054 PharmaGenie ELISA Kit SBRS2009
€760
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Description

Human and Mouse Phospho-TYK2 (Y1054) PharmaGenie ELISA - Kit Data

Product SKU:SBRS2009
Size:96T
Application:This ELISA kit recognizes Human.
Uniprot:P29597 / Q9R117
Gene ID:7297
Gene Names:TYK2
Pathway:Tyrosine Kinase Family
Synonyms:Non-receptor tyrosine-protein kinase TYK2 (EC 2.7.10.2)
Target Species:Human, Mouse
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.

Human and Mouse Phospho-TYK2 (Y1054) PharmaGenie ELISA Kit (SBES2009) - Product Images

Product ImageHeLa cells were treated with Calyculin A and IL-1 beta. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.
Product ImageHeLa cells were treated with Calyculin A. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Anti-Phospho Antibody
4.HRP-Conjugated Secondary Antibody
5.Assay Diluent
6.TMB One-Step Substrate
7.Stop Solution
8.Lysis Buffer
9.Positive Control Sample

Other materials and equipment required:

The Assay Genie Human and Mouse Phospho-TYK2 (Y1054) ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.