Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
The Human ANGPTL4 (Angiopoietin-like protein 4) ELISA Kit is specifically designed for the accurate quantification of ANGPTL4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.ANGPTL4 is a critical protein involved in the regulation of lipid metabolism, insulin sensitivity, and inflammation. Its dysregulation has been linked to various metabolic disorders, including obesity, diabetes, and cardiovascular diseases. As such, the measurement of ANGPTL4 levels can provide valuable insights into the development and progression of these conditions, making it a valuable tool for researchers and clinicians alike.Overall, the Human ANGPTL4 ELISA Kit is an essential resource for studying the role of ANGPTL4 in metabolism and disease pathogenesis, offering a reliable and efficient method for biomarker detection and analysis.
Product Name:
Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
SKU:
HUES01556
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
