The Human AR (Androgen Receptor) CLIA Kit offers a reliable method for the quantitative measurement of androgen receptor levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The androgen receptor is a key protein that plays a critical role in the regulation of androgen signaling, influencing a wide range of physiological processes including development, reproduction, and metabolism. Dysregulation of the androgen receptor has been implicated in various diseases such as prostate cancer, androgen insensitivity syndrome, and polycystic ovary syndrome, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. Overall, the Human AR (Androgen Receptor) CLIA Kit is an essential tool for researchers looking to study and characterize the role of the androgen receptor in health and disease, providing valuable insights into potential treatment strategies and diagnostic approaches.
Product Name:
Human AR (Androgen Receptor) CLIA Kit
SKU:
HUES00353
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.