The Human BECN1 (Beclin-1) ELISA Kit is specifically designed for the precise quantification of Beclin-1 levels in human biological samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.Beclin-1 is a key regulator of autophagy, a cellular process essential for maintaining homeostasis by degrading unnecessary or dysfunctional components. Dysregulation of autophagy has been implicated in various diseases including cancer, neurodegenerative disorders, and inflammatory conditions. Therefore, Beclin-1 serves as a critical biomarker for exploring disease mechanisms and potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human BECN1 ELISA Kit, providing you with the necessary tools to study autophagy and its role in health and disease. Visit the link above to learn more about this innovative product.
Product Name:
Human BECN1 (Beclin 1) ELISA Kit
SKU:
HUES01748
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
