The Human CLDN1 (Claudin-1) CLIA Kit is a powerful tool for studying the levels of CLDN1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.CLDN1, also known as Claudin-1, is a key protein involved in the formation and maintenance of tight junctions in epithelial and endothelial cells. Dysregulation of CLDN1 has been implicated in various diseases, including cancer, inflammatory bowel disease, and hepatitis.
Therefore, measuring CLDN1 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies.The Human CLDN1 CLIA Kit from Assay Genie offers researchers a convenient and efficient solution for quantifying CLDN1 levels, enabling deeper understanding of its role in health and disease. Discover the potential of CLDN1 as a biomarker with this cutting-edge CLIA kit.
Product Name:
Human CLDN1 (Claudin 1) CLIA Kit
SKU:
HUES00469
Target:
Human CLDN1 (Claudin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLDN1. You can calculate the concentration of Human CLDN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
91-105
94-108
Average (%)
107
96
100
1:4
Range (%)
85-98
92-109
93-108
Average (%)
92
100
100
1:8
Range (%)
95-107
88-101
98-111
Average (%)
102
94
105
1:16
Range (%)
99-116
91-102
95-110
Average (%)
106
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
97-108
103
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.24
311.52
795.72
92.5
327.0
852.76
Standard deviation
10.53
27.07
77.5
11.73
38.88
57.13
C V (%)
10.5
8.69
9.74
12.68
11.89
6.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLDN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN1 in samples. No significant cross-reactivity or interference between Human CLDN1 and analogues was observed.
