The CTSL ELISA Kit is designed for the quantitative detection of Cathepsin L (CTSL) levels in various human biological samples. CTSL is a critical protease enzyme implicated in a multitude of cellular activities, including protein degradation, cell growth, and apoptosis. It has a significant role in immunity, and its dysregulation can be associated with various pathological conditions such as cancer, cardiovascular disorders, and autoimmune diseases. Hence, precise detection of CTSL is crucial for understanding its role in these pathological mechanisms and for the development of targeted therapeutic strategies. Assay Genie's CTSL ELISA Kit offers exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures sturdy performance and is simple to use, making it an excellent choice for both research and clinical applications. Rely on Assay Genie's CTSL ELISA Kit for precise and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human CTSL (Cathepsin L) ELISA Kit
SKU:
AEES00028
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
