The Human DEFA3 (Neutrophil Defensin 3) ELISA Kit is a powerful tool for the precise measurement of DEFA3 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, offering valuable insights for a wide range of research purposes. DEFA3, also known as Neutrophil Defensin 3, is a key component of the innate immune system, playing a crucial role in defending against bacterial and fungal infections. Its dysregulation has been linked to various inflammatory conditions and autoimmune diseases, highlighting its importance as a potential diagnostic and therapeutic target in medical research.
By utilizing the Human DEFA3 (Neutrophil Defensin 3) ELISA Kit, researchers can uncover novel insights into the role of DEFA3 in health and disease, paving the way for the development of innovative treatment strategies and personalized medicine approaches. This kit is an indispensable resource for studying the complex mechanisms underlying immune responses and exploring new avenues for improving patient care.
Product Name:
Human DEFA3 (Neutrophil defensin 3) ELISA Kit
SKU:
HUFI05311
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-105%
91-103%
93-105%
EDTA Plasma (n = 5)
84-96%
87-100%
83-98%
Heparin Plasma (n = 5)
84-100%
80-100%
81-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-102
99
EDTA Plasma (n = 5)
88-99
93
Heparin Plasma (n = 5)
87-104
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
