The Human E-cad (E-cadherin) ELISA Kit from Assay Genie is a reliable and accurate tool for detecting E-cadherin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.E-cadherin is a critical protein involved in cell adhesion and epithelial-mesenchymal transition, playing a crucial role in tissue organization and development. Aberrant E-cadherin expression is associated with various diseases, including cancer and metastasis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. The Assay Genie Human E-cad ELISA Kit provides researchers with a powerful tool to accurately quantify E-cadherin levels, ultimately advancing our understanding of cellular processes and disease mechanisms. Upgrade your research with this high-quality ELISA kit from Assay Genie.
Product Name:
Human E-Cad (E-Cadherin) ELISA Kit
SKU:
HUES01301
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
