The Rat E-Cadherin (E-Cad) ELISA Kit is a specialized assay utilized for the quantitative measurement of E-Cadherin levels in various rat biological samples. E-Cadherin, a key cell adhesion molecule critical for maintaining tissue integrity and cell-cell adhesion, plays a vital role in cellular processes like cell differentiation, migration, and embryonic development. E-Cadherin functions as a tumor suppressor by regulating cell-cell adhesion and inhibiting cancer cell invasion and metastasis. Aberrant E-Cadherin expression is often associated with tumor progression, metastasis, and poor clinical outcomes in cancer patients. Quantifying E-Cadherin levels using this ELISA kit provides valuable insights into cell adhesion dynamics, tumor growth, and metastatic potential. The Rat E-Cad ELISA Kit offered by Assay Genie ensures accurate and reliable measurements of E-Cadherin levels in rat samples with exceptional sensitivity and specificity. Stringent quality control measures guarantee robust performance, reproducible results, and user-friendly protocols, making this kit an excellent choice for researchers investigating the role of E-Cadherin in cancer biology, tissue development, and cell behavior in various experimental settings.
Product Name:
Rat E-Cad (E-Cadherin) ELISA Kit
SKU:
AEES00385
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
