The Mouse E-Cad (E-Cadherin) ELISA Kit is specifically designed for the accurate measurement of E-Cadherin levels in mouse serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring consistent and reliable results for various research applications.E-Cadherin is a key protein involved in cell adhesion and tissue organization, influencing processes such as embryonic development, wound healing, and tumor progression. Dysregulation of E-Cadherin has been linked to various diseases including cancer, making it an important biomarker for understanding disease pathology and potential therapeutic interventions. This ELISA kit provides researchers with a valuable tool for studying E-Cadherin dynamics in mouse models, offering insights into cellular behavior, disease mechanisms, and potential treatment strategies. With its high performance and ease of use, the Mouse E-Cad ELISA Kit is an essential resource for advancing scientific discovery in the field of molecular biology and pathology.
Product Name:
Mouse E-Cad (E-Cadherin) ELISA Kit
SKU:
MOES00788
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
