Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
The Human ECF (CCL11/Eosinophil Chemotactic Factor) ELISA Kit is a robust and reliable tool for the quantitative measurement of ECF levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ECF, also known as CCL11, is a chemokine that plays a crucial role in recruiting eosinophils to sites of inflammation and is involved in allergic responses. Elevated levels of ECF have been linked to conditions such as asthma, allergic rhinitis, and inflammatory diseases, making it a valuable biomarker for studying these conditions and evaluating treatment responses. With its advanced technology and user-friendly design, the Human ECF ELISA Kit from Assay Genie provides researchers with a powerful tool for studying the role of ECF in various physiological and pathological processes. Order yours today and unlock the potential of ECF research.
Product Name:
Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
SKU:
HUES01312
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
