The Human F1+2 Prothrombin Fragment 1+2 ELISA Kit is specifically designed for the accurate measurement of F1+2 Prothrombin Fragment 1+2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.F1+2 Prothrombin Fragment 1+2 is a key marker of thrombin generation and activation of the coagulation cascade. It is involved in blood clotting processes and is crucial in conditions such as thrombosis, hemophilia, and other coagulation disorders. This makes it a valuable biomarker for understanding these conditions and developing targeted treatments.Get reliable and reproducible data with the Human F1+2 Prothrombin Fragment 1+2 ELISA Kit, a powerful tool for researchers studying coagulation disorders and related pathologies. Order yours today and uncover valuable insights in your research.
Product Name:
Human F1+2 (Prothrombin Fragment 1+2) ELISA Kit
SKU:
HUES02761
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
