The Human F8 (Coagulation Factor 8) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Factor 8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a wide range of research applications.Factor 8 is a crucial protein involved in the coagulation pathway, playing a key role in blood clot formation.
Dysregulation of Factor 8 levels can lead to bleeding disorders such as hemophilia A, making it a critical biomarker for the diagnosis and monitoring of these conditions. The Human F8 CLIA Kit allows for the precise quantification of Factor 8 levels, providing valuable insights into the coagulation process and aiding in the development of novel therapeutic strategies.
Product Name:
Human F8 (Coagulation Factor 8) CLIA Kit
SKU:
HUES00522
Target:
Human F8 (Coagulation Factor 8)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F8. You can calculate the concentration of Human F8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
93-106
86-100
Average (%)
93
100
93
1:4
Range (%)
95-108
88-102
84-99
Average (%)
101
94
91
1:8
Range (%)
94-107
98-112
86-97
Average (%)
100
103
91
1:16
Range (%)
91-108
102-120
92-107
Average (%)
99
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.11
377.5
1679.54
230.89
360.33
1787.94
Standard deviation
23.39
31.94
144.1
19.26
32.25
132.49
C V (%)
11.08
8.46
8.58
8.34
8.95
7.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
