Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit
The Human FGF23 ELISA Kit is specifically designed for the precise measurement of FGF23 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.FGF23 is a vital protein that plays a crucial role in phosphate and vitamin D metabolism, as well as in bone mineralization. Dysregulation of FGF23 has been linked to several diseases, including chronic kidney disease, hypophosphatemic rickets, and certain types of cancer. Monitoring FGF23 levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human FGF23 ELISA Kit is an indispensable tool for researchers studying FGF23-related disorders and seeking to understand the mechanisms underlying these diseases. Its high performance and reliability make it an excellent choice for researchers in the field of medical and biological sciences.
Product Name:
Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit
SKU:
HUES02207
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
