Human FNDC5 (Fibronectin type III domain-containing protein 5) ELISA Kit
The Human FNDC5 (Fibronectin type III domain-containing protein 5) ELISA Kit is a state-of-the-art assay designed to quantitatively measure FNDC5 protein levels in human samples. FNDC5, a key regulator of metabolism and energy expenditure, plays a crucial role in modulating physiological processes such as mitochondrial biogenesis and thermogenesis. Understanding the expression levels of FNDC5 can provide valuable insights into metabolic homeostasis, exercise-induced adaptations, and potential therapeutic targets for metabolic disorders. With exceptional sensitivity and specificity, the Human FNDC5 ELISA Kit enables researchers to accurately assess FNDC5 protein levels, offering a deeper understanding of its metabolic functions and potential implications in the pathophysiology of metabolic diseases. By providing precise measurements of FNDC5, this ELISA kit empowers researchers to explore the intricate mechanisms underlying energy metabolism, exercise physiology, and metabolic health. Manufactured to stringent quality control standards, the Human FNDC5 ELISA Kit delivers reliable and reproducible results, ensuring robust performance in metabolic research studies. Researchers investigating FNDC5-mediated metabolic pathways and metabolic disorders can leverage this user-friendly kit to advance their research and contribute to the knowledge of FNDC5-related metabolic processes.
Product Name:
Human FNDC5 (Fibronectin type III domain-containing protein 5) ELISA Kit
SKU:
AEES00670
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
