The Hepatitis B virus surface antigen (HBsAg) is a key marker of Hepatitis B viral infection. It is a protein located on the outer surface of the Hepatitis B virus and is involved in viral entry and immune recognition. The presence of HBsAg in the bloodstream indicates an active Hepatitis B infection and can be used for diagnosis and monitoring purposes. The Assay Genie Human Hepatitis B virus surface antigen ELISA Kit is a highly sensitive assay designed for the quantitative measurement of HBsAg in serum, plasma, cell culture supernatant, and tissue samples.
Description
Human HbsAg (Hepatitis B virus Surface Antigen) ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human HbsAg (hepatitis B virus Surface Antigen) ELISA Kit |
| Product Code: | HUFI08043 |
| Size: | 96 Assays |
| Alias: | HbsAg ELISA Kit |
| Detection Method: | Qualitative ELISA |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human HbsAg (hepatitis B virus Surface Antigen) concentrations in serum plasma and other biological fluids. |
| Sensitivity: | Contact Technical Support |
| Range: | Contact Technical Support |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Human HbsAg (hepatitis B virus Surface Antigen) and the recovery rates were calculated by comparing the measured value to the expected amount of Human HbsAg (hepatitis B virus Surface Antigen) in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human HbsAg (hepatitis B virus Surface Antigen) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 2-8°C |
| Negative Control | 1 vial | 2-8°C |
| Positive Control | 1 vial | 2-8°C |
| Sample Dilution Buffer | 1 vial | 4°C (Protection from light) |
| HRP conjugated antibody | 1 vial | 2-8°C |
| Wash Buffer (20X) | 2 x 25 mL | 2-8°C |
| Substarte A | 1 vial | 4°C |
| Substrate B | 1 vial | 4°C (Protection from light) |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
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