The Human I-PTH (intact Parathormone) ELISA Kit is meticulously crafted for the precise and quantitative detection of intact Parathormone in a variety of biological samples. Intact Parathormone plays a fundamental role in calcium and phosphorus metabolism by regulating calcium levels in the blood through its actions on the bones, kidneys, and intestines. This ELISA kit allows for accurate measurement of intact Parathormone levels, providing valuable insights into bone health, vitamin D metabolism, and calcium homeostasis. With exceptional sensitivity and specificity, the kit ensures reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust the Human I-PTH ELISA Kit for accurate and dependable quantification of this essential biomarker in your studies.
Product Name:
Human I-PTH (intact Parathormone) ELISA Kit
SKU:
AEES00112
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
