The Human IFNα (Interferon Alpha) ELISA Kit is expertly designed to facilitate the quantitative measurement of Interferon Alpha in diverse human biological samples. Interferon Alpha plays a vital role in the body's immune response, particularly in combating viral infections and regulating immune cell activities. It serves as a critical cytokine with implications in antiviral defense, immune modulation, and overall immune system function. Our ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results essential for elucidating the role of Interferon Alpha in immune responses and immune-related disorders. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly protocols, making it an invaluable tool for research applications. Trust in Assay Genie's IFNα ELISA Kit for dependable quantification and thorough exploration of this key biomarker in your research studies.
Product Name:
Human IFN alpha (Interferon Alpha) ELISA Kit
SKU:
AEES00153
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
