The Rat IFNα (Interferon Alpha) ELISA Kit is a specialized assay designed for the quantitative analysis of Interferon Alpha levels in a variety of biological samples collected from rat models. Interferon Alpha is a vital cytokine involved in the regulation of immune responses, particularly in viral infections and autoimmune diseases, serving as a key mediator in antiviral defense mechanisms and immune modulation. This ELISA kit provides researchers with a reliable method to measure and quantify the levels of Interferon Alpha, enabling detailed investigations into its role in immune regulation, antiviral immunity, and inflammatory responses. Accurate measurement of IFNα is essential for understanding its functions in various disease conditions and immune system activities. Manufactured under strict quality control guidelines, the Rat IFNα ELISA Kit ensures exceptional sensitivity and specificity, allowing for consistent and reproducible results. Its robust performance and user-friendly design make it an excellent choice for research studies focused on immune response modulation and inflammatory processes in rat models.
Product Name:
Rat IFN alpha (Interferon Alpha) ELISA Kit
SKU:
AEES00549
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
