Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit
The Human IGFBP-3 (Insulin-like Growth Factor Binding Protein-3) ELISA Kit is a reliable tool for the precise measurement of IGFBP-3 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and consistent results for various research applications.IGFBP-3 is a critical regulator of insulin-like growth factor (IGF) signaling, modulating the bioavailability and activity of IGFs. It plays a crucial role in cell growth, differentiation, and apoptosis, making it a valuable biomarker in studies related to cancer, diabetes, and growth disorders. By accurately quantifying IGFBP-3 levels, researchers can gain insights into its role in disease pathogenesis and potentially identify new therapeutic targets.Overall, the Human IGFBP-3 ELISA Kit offers researchers a dependable tool for investigating the complex interplay between IGFBP-3 and IGF signaling pathways, ultimately advancing our understanding of various physiological and pathological processes.
Product Name:
Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit
SKU:
HUES01373
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
