The Human IgG1 (Immunoglobulin G1) ELISA Kit is a highly sensitive assay designed for the accurate quantification of IgG1 levels in human biological samples. Immunoglobulin G1 is a major subclass of antibodies that plays a critical role in the humoral immune response, providing defense against pathogens, toxins, and foreign agents. This ELISA kit offers a reliable method for researchers to measure IgG1 concentrations, facilitating the investigation of immune function, antibody responses, and disease pathogenesis in human samples. IgG1 antibodies are known for their versatility in immune defense, including opsonization, neutralization, and antibody-dependent cell-mediated cytotoxicity. By detecting and quantifying IgG1 levels, researchers can gain insights into immune system activity, immunity to specific antigens, and antibody-mediated responses in various diseases and conditions. This ELISA kit provides a precise and reproducible means to assess IgG1 levels, supporting studies on immune status, vaccine response, and autoimmune disorders in research and clinical settings. The Human IgG1 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring accurate and trustworthy results. Manufactured with stringent quality control measures, this kit delivers robust performance and user-friendly protocols, offering researchers a valuable tool for immunology research, diagnostic investigations, and antibody profiling studies in human samples.
Product Name:
Human IgG1 (Immunoglobulin G1) ELISA Kit
SKU:
AEES00651
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
5.63 μg/mL
Detection range:
9.38-600 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
