The Rat IgG1 (Immunoglobulin G1) ELISA Kit is designed for the quantitative detection of Rat IgG1 (Immunoglobulin G1) levels in various biological samples. Immunoglobulin G1 is a biologically active molecule with established roles in immunology, cell biology pathways. It participates in key cellular and molecular processes that regulate physiological homeostasis, and dysregulation of its expression or activity has been associated with pathological states across multiple disease contexts. The precise quantification of Rat Immunoglobulin G1 in biological samples is critical for elucidating its mechanistic contributions to disease pathogenesis, identifying clinically relevant biomarker associations, and supporting the development of novel diagnostic tools and targeted therapeutic interventions. Assay Genie's Rat IgG1 (Immunoglobulin G1) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 0.19 μg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Rat IgG1 (Immunoglobulin G1) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Rat IgG1 (Immunoglobulin G1) ELISA Kit
SKU:
AEES03532
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
