The Human INHB (Inhibin B) ELISA Kit is specially designed for the precise measurement of inhibin B levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and dependable results, making it a valuable tool for various research purposes.Inhibin B is a key hormone that plays a critical role in regulating the reproductive system by inhibiting the production of follicle-stimulating hormone (FSH). Imbalances in inhibin B levels are associated with infertility, polycystic ovary syndrome (PCOS), and other reproductive disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.With the Human INHB ELISA Kit, researchers can accurately measure inhibin B levels in human samples, advancing their understanding of reproductive health and paving the way for new diagnostic and treatment strategies.
Product Name:
Human INHB (Inhibin B) ELISA Kit
SKU:
HUES01537
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.