Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Human IP-10 (CXCL10) Interferon Gamma-Induced Protein 10kDa ELISA Kit is a cutting-edge tool designed for the precise measurement of IP-10 levels in human serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it the perfect choice for a diverse array of research endeavors.IP-10, also known as CXCL10, is a critical chemokine involved in the immune response, particularly in the recruitment of T cells to sites of inflammation. It is induced by interferon-gamma and plays a key role in the pathogenesis of various diseases, including autoimmune disorders and infectious diseases. Monitoring IP-10 levels can provide valuable insights into immune responses and disease progression, making it a valuable biomarker for research and potential therapeutic interventions.
Product Name:
Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
HUES01337
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
85-100
99-114
90-103
Average (%)
92
104
95
1:4
Range (%)
92-107
86-101
97-110
Average (%)
100
93
103
1:8
Range (%)
96-108
86-100
97-112
Average (%)
103
93
104
1:16
Range (%)
94-111
87-100
92-103
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
93-111
101
Cell Culture Media (n=8)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.2
58.25
222.73
25.44
53.19
223.57
Standard deviation
1.5
3.74
15.44
1.91
3.24
19.34
C V (%)
5.51
6.42
6.93
7.49
6.09
8.65
Uniport ID:
P02778
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed
