Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
The Human KRT10 (Keratin Type I Cytoskeletal 10) CLIA Kit is a reliable and accurate tool for measuring levels of KRT10 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research studies.KRT10 is a key protein involved in maintaining the structure and integrity of keratinocytes in the skin. Changes in KRT10 levels have been associated with various skin disorders, making it a valuable biomarker for studying skin diseases and developing therapeutic interventions.Overall, the Human KRT10 CLIA Kit is essential for researchers in the fields of dermatology, cell biology, and molecular biology who are interested in studying the role of KRT10 in skin health and disease.
Product Name:
Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
SKU:
HUES01129
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
