The Human LYZ (Lysozyme) ELISA Kit is specifically designed for the sensitive and accurate detection of lysozyme levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Lysozyme is an important enzyme that plays a key role in the immune system by breaking down bacterial cell walls. It is also involved in various physiological processes, such as wound healing and digestion. Dysregulation of lysozyme levels has been associated with inflammatory diseases, infections, and certain cancers, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human LYZ (Lysozyme) ELISA Kit provides researchers with a powerful tool for exploring the role of lysozyme in health and disease, offering valuable insights into potential diagnostic and treatment options.
Product Name:
Human LZM (Lysozyme) ELISA Kit
SKU:
HUES02821
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
