The Human MBP (Myelin Basic Protein) CLIA Kit is specifically designed for the precise and reliable measurement of MBP levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for various research applications.Myelin Basic Protein is a key component of the myelin sheath, essential for the proper functioning of the nervous system. Abnormal levels of MBP are associated with neurological disorders such as multiple sclerosis and other demyelinating diseases.
Studying MBP levels can provide valuable insights into these conditions and aid in the development of potential therapies.Overall, the Human MBP CLIA Kit is a valuable tool for researchers looking to investigate the role of MBP in neurological disorders and explore its potential as a biomarker for disease diagnosis and treatment.
Product Name:
Human MBP (Myelin Basic Protein) CLIA Kit
SKU:
HUES00141
Target:
Human MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MBP. You can calculate the concentration of Human MBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
93-107
98-112
Average (%)
99
100
105
1:4
Range (%)
96-112
95-111
95-108
Average (%)
103
103
101
1:8
Range (%)
90-100
101-117
97-110
Average (%)
95
109
105
1:16
Range (%)
94-112
94-108
86-99
Average (%)
102
101
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
105
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.38
73.46
184.79
23.96
70.98
187.74
Standard deviation
3.23
7.4
11.96
2.67
7.61
21.06
C V (%)
12.24
10.07
6.47
11.14
10.72
11.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MBP in samples. No significant cross-reactivity or interference between Human MBP and analogues was observed.
