Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit
The Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit is expertly designed to enable the precise quantitative analysis of Macrophage Migration Inhibitory Factor (MIF) levels in a variety of biological samples. MIF is a pivotal pro-inflammatory cytokine known for its multifaceted roles in modulating immune responses, cell migration, and inflammation processes. It serves as a critical regulator of the body's physiological responses to various stressors, infections, and diseases, thereby influencing inflammatory cascades and immune cell activities. Our MIF ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible measurement of MIF concentrations. With stringent quality control protocols in place, this kit delivers robust and reliable performance, making it an ideal choice for research applications. Trust Assay Genie's MIF ELISA Kit for dependable quantification of this essential cytokine in your research endeavors.
Product Name:
Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit
SKU:
AEES00171
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
