The Human MUC1 (Mucin 1) ELISA Kit is specifically designed for the precise measurement of Mucin 1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Mucin 1, also known as MUC1, is a glycoprotein that plays a critical role in cell signaling, adhesion, and immune response regulation. Abnormal Mucin 1 expression has been associated with various diseases, including cancer, inflammatory disorders, and infectious diseases. Therefore, the Human MUC1 ELISA Kit serves as a valuable tool for studying Mucin 1-related pathologies and developing potential therapeutic strategies.
Product Name:
Human MUC1 (Mucin 1) ELISA Kit
SKU:
HUES01780
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
