The Human MUC5B (Mucin 5 Subtype B) ELISA Kit is specifically designed for the precise measurement of MUC5B levels in human samples including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MUC5B is a key mucin protein involved in mucin production and regulation, particularly in the respiratory tract where it plays a crucial role in maintaining mucosal integrity and protecting against pathogens. Dysregulation of MUC5B expression has been linked to respiratory disorders such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and asthma, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions. Overall, the Human MUC5B ELISA Kit is a valuable tool for researchers looking to investigate the role of MUC5B in respiratory health and disease, offering reliable results and insights that can contribute to advancements in respiratory medicine and therapeutics.
Product Name:
Human MUC5B (Mucin 5 Subtype B) ELISA Kit
SKU:
HUES03163
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
