The Mouse MUC5B (Mucin 5 Subtype B) ELISA Kit is specifically designed for the precise measurement of mouse MUC5B levels in biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.MUC5B is a key mucin protein that plays a vital role in the respiratory system by providing protection and lubrication to the airway epithelium. Dysregulation of MUC5B expression has been associated with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and asthma, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions. With its reliable performance and ease of use, the Mouse MUC5B ELISA Kit is an indispensable tool for researchers investigating mucin biology and respiratory diseases in preclinical and clinical settings.
Product Name:
Mouse MUC5B (Mucin-5 Subtype B) ELISA Kit
SKU:
MOES01276
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
