Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
The Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is specifically designed for the quantitative detection of NOS2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.NOS2, also known as iNOS, is an enzyme that plays a key role in the production of nitric oxide, a molecule involved in various physiological and pathological processes. Abnormal NOS2 expression has been linked to inflammatory diseases, neurodegenerative disorders, and cancer, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Human NOS2/iNOS ELISA Kit is an essential tool for researchers looking to investigate the role of NOS2 in health and disease. Order yours today and unlock the potential of NOS2 research.
Product Name:
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
SKU:
HUES01897
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
