The Human NPHN (Nephrin) ELISA Kit is specifically designed for the precise measurement of nephrin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes. Nephrin is a key protein involved in maintaining the structure and function of the kidney's filtration barrier. Changes in nephrin levels have been linked to various kidney diseases, including diabetic nephropathy and glomerulonephritis. By measuring nephrin levels accurately, researchers can gain valuable insights into the pathogenesis of these conditions and explore potential therapeutic interventions. With its reliable performance and broad applicability, the Human NPHN (Nephrin) ELISA Kit is an indispensable tool for studying kidney-related disorders and advancing research in the field of nephrology.
Product Name:
Human NPHN (Nephrin) ELISA Kit
SKU:
HUES02849
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
