The Human PCIII (Procollagen III) ELISA Kit is specifically designed for the precise measurement of Procollagen III levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research applications.Procollagen III is an important precursor protein involved in collagen synthesis, playing a key role in maintaining skin elasticity and overall tissue structure. Imbalances in Procollagen III levels have been linked to conditions such as liver fibrosis, arthritis, and dermatological disorders, making it a valuable biomarker for understanding these diseases and exploring potential therapies.This ELISA kit provides researchers with a reliable tool for studying Procollagen III levels in human samples, offering a deep insight into the underlying mechanisms of these conditions and paving the way for innovative treatment strategies.
Product Name:
Human PCIII (Procollagen III) ELISA Kit
SKU:
HUES01449
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
