Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
The Human PDGF (Platelet-Derived Growth Factor) AB ELISA Kit is specifically designed for the precise measurement of PDGF levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PDGF is a key growth factor known for its role in promoting cell growth, proliferation, and differentiation. It plays a crucial function in wound healing, tissue repair, and development of various organs. Dysregulation of PDGF signaling has been implicated in conditions such as cancer, fibrosis, and vascular diseases, highlighting its importance as a biomarker for disease progression and potential therapeutic targets.With reliable performance and easy-to-follow protocols, the Human PDGF AB ELISA Kit from Assay Genie is an essential tool for researchers investigating the role of PDGF in health and disease, providing valuable insights into mechanisms of growth regulation and potential treatment strategies.
Product Name:
Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
SKU:
HUES02587
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
