Human Phosphotyrosine EphB1 PharmaGenie ELISA Kit (SBRS1809)

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Human Phosphotyrosine EphB1 PharmaGenie ELISA - Kit Data

Product SKU:SBRS1809
Application:The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphB1.
Gene ID:2047
Gene Names:ELK / EPHB1 / EPHT2 / HEK6 / NET
Pathway:Tyrosine Kinase Family
Synonyms:Ephrin type-B receptor 1 (EC (ELK) (EPH tyrosine kinase 2) (EPH-like kinase 6) (EK6) (hEK6) (Neuronally-expressed EPH-related tyrosine kinase) (NET) (Tyrosine-protein kinase receptor EPH-2)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Storage/Stability:Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Biotinylated Anti-Phosphotyrosine Antibody
4.Stop Solution
5.Assay Diluent(s)
6.Positive Control Sample
7.Lysis Buffer
8.Streptavidin-Conjugated HRP
9.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Phosphotyrosine EphB1 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.