Description
Human Phosphotyrosine SCFR PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS1959 |
| Size: | 96T |
| Application: | The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-SCFR. |
| Uniprot: | P10721 |
| Gene ID: | 3815 |
| Gene Names: | KIT / SCFR |
| Pathway: | Tyrosine Kinase Family |
| Synonyms: | Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Piebald trait protein) (PBT) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (p145 c-kit) (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (CD antigen CD117) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Lysates, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Storage/Stability: | Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C. |
Human Phosphotyrosine SCFR PharmaGenie ELISA Kit (SBES1959) - Product Images
 | HeLa cells were treated or untreated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA: |
 | JURKAT cells were treated with Pervanadate at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Biotinylated Anti-Phosphotyrosine Antibody |
| 4. | Stop Solution |
| 5. | Assay Diluent(s) |
| 6. | Positive Control Sample |
| 7. | Lysis Buffer |
| 8. | Streptavidin-Conjugated HRP |
| 9. | TMB One-Step Substrate |
Other materials and equipment required:
The Assay Genie Human Phosphotyrosine SCFR ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Kit Protocol:
| 1. | Prepare all reagents and samples as instructed in the manual. |
| 2. | Add 100 ul of sample or positive control to each well. |
| 3. | Incubate 2.5 h at RT or O/N at 4 °C. |
| 4. | Add 100 ul of prepared primary antibody to each well. |
| 5. | Incubate 1 h at RT. |
| 6. | Add 100 ul of prepared 1X HRP-Streptavidin to each well. |
| 7. | Incubate 1 h at RT. |
| 8. | Add 100 ul of TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul of Stop Solution to each well. |
| 11. | Read at 450 nm immediately. |
