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Human Phosphotyrosine TIE-1 PharmaGenie ELISA Kit (SBRS2002)

SKU:
SBRS2002
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phosphotyrosine TIE-1 PharmaGenie ELISA Kit SBRS2002
  • Human Phosphotyrosine TIE-1 PharmaGenie ELISA Kit SBRS2002
€649
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Description

Human Phosphotyrosine TIE-1 PharmaGenie ELISA Kit (SBRS2002)

Product SKU:SBRS2002
Size:96T
Application:This ELISA kit recognizes human TIE-1 phosphotyrosine.
Uniprot:P35590
Gene ID:7075
Gene Names:TIE / TIE1
Pathway:Tyrosine Kinase Family
Synonyms:Tyrosine-protein kinase receptor Tie-1 (EC 2.7.10.1)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Biotinylated Anti-Phosphotyrosine Antibody
  4. Stop Solution
  5. Assay Diluent(s)
  6. Positive Control Sample
  7. Lysis Buffer
  8. Streptavidin-Conjugated HRP
  9. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Phosphotyrosine TIE-1 PharmaGenie ELISA Kit (SBRS2002) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageK562 cells were untreated or treated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA:
Product ImageK562 cells were treated with Pervanadate. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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