Human P1NP (Procollagen I N-terminal Propeptide) ELISA Kit
The Human PINP (Procollagen I N-Terminal Propeptide) ELISA Kit is specifically designed for the quantitative detection of PINP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PINP is a key marker for collagen synthesis and bone formation, serving as a vital indicator of bone turnover and health. Elevated PINP levels are associated with conditions like osteoporosis, fractures, and other bone diseases, making it a valuable biomarker for studying bone metabolism and assessing the efficacy of therapeutic interventions. With its reliable performance and user-friendly protocols, the Human PINP ELISA Kit is an essential tool for researchers and healthcare professionals seeking to study bone health, monitor disease progression, and develop targeted treatment strategies.
Product Name:
Human P1NP (Procollagen I N-terminal Propeptide) ELISA Kit
SKU:
HUES01452
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
